Quantitative Test Of Quercetin Content Of Rome Beauty Apple Flour Extract Using The High-Performance Liquid Chromatography Method

Abstract

Introduction: Apple is a fruit containing antioxidant phytochemicals, one of which is quercetin, a flavonoid compound. Apples are one of the natural ingredients that have a beneficial effect on health. Rome beauty apples (Malus sylvestris Mill.) are natural antioxidants that are safer for consumption compared to synthetic antioxidants, which have the potential to cause harm. Oxidative stress occurs due to an imbalance in the amount of antioxidants in the body, causing cell damage. High-Performance Liquid Chromatography (HPLC) was conducted to determine the antioxidant content, especially quercetin, in Rome Beauty apple extract.

Objective: to find out how to quantitatively test the quercetin flavonoids from Rome Beauty apple extract (Malus sylvestris Mill.) using HPLC to determine the levels of quercetin flavonoids in Rome Beauty apple extract (Malus sylvestris Mill.).

Method: The method of sample preparation was carried out using a maceration extraction technique with 70% ethanol solvent. Determination of the quercetin content in Rome Beauty Apple Extract was carried out using the High-Performance Liquid Chromatography (HPLC) method and a UV spectrophotometer detector.

Results: The results of the quantitative tests that have been carried out conclude that Rome Beauty apple extract (Malus sylvestris Mill.) has a quercetin content of 557.999 ppm and a quercetin content of 0.557%.

Conclusion: Rome Beauty apples (Malus sylvestris Mill.) positively contain the flavonoid compound quercetin