Acute toxicity test of probiotic red guava, apple, lemon, melon juice with bacteria starter Lactobacillus acidophilus on male Balb/c. Mice

Abstract

Introduction: Before a new product candidate for a functional drink can be produced and distributed as a supplement that is beneficial to the body, it is necessary to test its safety for consumption by humans, which includes the selection stages, namely preclinical testing, one of which is an acute in vivo toxicity test. Purpose: This study was to determine whether or not a toxic effect occurred after the administration of probiotic drinks. In doses of 5, 50, 300, and 2000 mg/KgBB. Methods: The study used an experimental design with a quantitative research type using the oxidation method, namely the Fixed Dose Method. Fifteen male mice (Balb/c) were used as test animals divided into 5 groups. Probiotic drinks were administered orally at 5, 50, 300, and 2000 mg/KgBW doses, and distilled water was used as the control group. The test animals were observed individually for 14 days of observation. The parameters observed were symptoms of toxicity and the number of deaths that occurred. Results: The results of the Kruskal Wallis analysis showed no significant difference in the number of deaths caused by the administration of probiotics. Toxicity symptoms
occurred in the group treated with distilled aqua. (Toxic symptoms include hair loss, trembling, and walking on the stomach. Conclusion: From this study, giving juice probiotics with L.acidophillus starter and doses of 5, 50, 300, and 2000 mg/KgBW did not cause death and symptoms of toxicity were changed in male balb/c mice observed for 14 days.