Acute Toxicity Test of Probiotic Juice Red Guava, Lemon, Melon, and Apple With Bacteria Starter Lactobacillus casei on Female Balb/C Mice

Abstract

Introduction: Probiotic drinks are drinks that have health effects and contain living microbes in them. These drinks are popular among the public, and have started to consume these drinks. Probiotic drinks contain probiotic agents made through fermentation, one of which is Lactobacillus casei. Before consumption, probiotic drinks will undergo preclinical testing, one of which is in vivo. This study aimed to determine if the purpose was to determine if there was an acute toxicity effect after being given a juice probiotic drink. Methods: This quantitative research type applied research applied research was a quantitative research type applying an experimental study design. The variables used are independent and dependent. This study used 15 female white mice (Balb/c) divided into five groups. The test material used was the Probiotic drink, administered orally at 5, 50,300, and 2000 mg/KgBW doses and distilled water as the control group. The test animals were observed individually for 14 days. The variables observed were clinical symptoms like seizures, saliva, tremors, limp, and walking using the stomach. Results: There are no toxic symptoms that occurred in mice Balb/C like seizures, saliva, tremors, limp, and walking using the stomach during 14 days of observation. Conclusion: There were no clinical symptoms that occurred in the toxicity test. This study concluded that probiotic drink juice with L.casei starter at doses of 5, 50, 300, and 2000 mg/KgBW did not cause a decrease in no toxic symptoms occurred in mice Balb/C.